Human Granzyme A (Gzms-A) ELISA Kit

Cat.#:

JL10492

Regulatory Status:

RUO

Alternative:

CTLA3; HFSP; HF; H factor; Granzyme 1; Fragmentin-1; Hanukkah factor; Cytotoxic T-Lymphocyte-Associated Serine Esterase 3; CTL tryptase; Cytotoxic T-lymphocyte proteinase 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.06ng/mL

Specificity:

This kit recognizes Human Gzms-A in samples. No significant cross-reactivity or interference between Human Gzms-A and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Procedure:

Research Area:

Cell Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| Gzms-A

Granzyme A (Gzms-A, EC 3.4.21.78, CTLA3, HuTPS, T-cell associated protease 1, cytotoxic T lymphocyte serine protease, TSP-1, T-cell derived serine proteinase) is a tryptase[5][6][7] and is one of the five granzymes encoded in the human genome.In humans, GzmA is encoded by the GZMA gene in proximity to the GZMK gene on chromosome 5. This enzyme is present in cytotoxic T lymphocyte (CTL) granules.Cytolytic T lymphocytes (CTL) and natural killer (NK) cells have a remarkable ability to recognize, bind, and lyse specific target cells.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.15	0
OD	2.4	1.69	1.29	0.9	0.61	0.43	0.31	0.09
Corrected OD	2.31	1.6	1.2	0.81	0.52	0.34	0.22	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human Gzms-A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Lysates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human Gzms-A and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-109	105-115
1 : 4	Average (%)	94	98	108